https://nova.newcastle.edu.au/vital/access/ /manager/Index ${session.getAttribute("locale")} 5 https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:15929 Wed 11 Apr 2018 16:27:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:27947 Wed 11 Apr 2018 14:50:35 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:17231 K,L. A similar current was first observed at 15 WG but remained relatively small, even at 18 WG. The presence of a “collapsing” tail current indicates a maturing type I hair cell phenotype and suggests the presence of a surrounding calyx afferent terminal. We were also able to record from calyx afferent terminals in 15–18 WG cristae. In voltage clamp, these terminals exhibited fast inactivating inward as well as slower outward conductances, and in current clamp, discharged a single action potential during depolarizing steps. Together, these data suggest the major functional characteristics of type I and type II hair cells and calyx terminals are present by 18 WG. Our study also describes a new preparation for the functional investigation of key events that occur during maturation of human vestibular organs.]]> Wed 11 Apr 2018 14:41:28 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:14888 Wed 11 Apr 2018 14:03:26 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:48765 Wed 05 Apr 2023 13:55:44 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:53981 Thu 25 Jan 2024 12:57:12 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:44697 Thu 20 Oct 2022 09:51:36 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:42126 Thu 18 Aug 2022 15:03:24 AEST ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:21485 Salmonella enteritidis) or saline on postnatal days (PNDs) 3 and 5 and later subjected to the formalin test at PNDs 7, 13, and 22. One hour after formalin injection, blood was collected to assess corticosterone responses. Transverse spinal cord slices were also prepared for whole-cell patch clamp recording from lumbar superficial dorsal horn neurons (SDH). Brains were obtained at PND 22 and the hypothalamus was isolated to measure glucocorticoid (GR) and mineralocorticoid receptor (MR) transcript expression using qRT-PCR. Behavioural analyses indicate that at PND 7, no significant differences were observed between saline- or LPS-challenged rats. At PND 13, LPS-challenged rats exhibited enhanced licking (p < .01), and at PND 22, increased flinching in response to formalin injection (p < .05). LPS-challenged rats also displayed increased plasma corticosterone at PND 7 and PND 22 (p < .001) but not at PND 13 following formalin administration. Furthermore, at PND 22 neonatal LPS exposure induced decreased levels of GR mRNA and increased levels of MR mRNA in the hypothalamus. The intrinsic properties of SDH neurons were similar at PND 7 and PND 13. However, at PND 22, ipsilateral SDH neurons in LPS-challenged rats had a lower input resistance compared to their saline-challenged counterparts (p < .05). These data suggest neonatal LPS exposure produces developmentally regulated changes in formalin-induced behavioural responses, corticosterone levels, and dorsal horn neuron properties following noxious stimulation later in life. These findings highlight the importance of immune activation during the neonatal period in shaping pain sensitivity later in life. This programming involves both spinal cord neurons and the HPA axis.]]> Sat 24 Mar 2018 08:03:35 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:47389 Mon 16 Jan 2023 15:45:43 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:54764 Mon 11 Mar 2024 15:00:48 AEDT ]]> https://nova.newcastle.edu.au/vital/access/ /manager/Repository/uon:39675 Fri 17 Jun 2022 15:32:12 AEST ]]>